| Solution Information | help | |
| Enzyme: | Amyloid-beta A4 precursor protein-binding family A member 1 | |
| inhibitor: | BDBM71548 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay materials: 1) Biotin, glutathione S-transferase (GST)-fusion protein (GST-M15) and biotinylated (biotin-) N-type Ca2+ channel carboxyl-terminal peptide (biotin-NC peptide) corresponding to the carboxyl-terminal tail of CaV2.2 protein were supplied by assay provider. 2) Anti-GST monoclonal antibody labeled with europium cryptate (EuK-anti-GST-mAb (CisBio cat# 61GSTKLB) and streptavidin-XL665 (CisBio cat# 610SAXLB ). 3) Potassium Fluoride (KF) (Aqua Solutions cat# P3901) 4) Tween-20 (Sigma cat# 9416) 5) Bovine Serum Albumin (Sigma cat# A7888) Working solutions: 1. Assay buffer: 25 mM HEPES, pH 7.2, 100mM NaCl,0.1% BSA, 0.005% Tween-20 2. Mint1-PDZ working solution (1.75X final concentration) contains 3.5 nM GST-MINT-PDZ and 0.63 nM EuK-anti-GST-mAb 3. Biotin-NC peptide-XL665 solution (3.5X final concentration) contained 167 nM biotin-NC peptide and 46.6 nM streptavidin XL665. 4. Biotin solution (3.5X final concentration): 167 nM Biotin 3.5X final concentration) and 46.6 nM str | |
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